DWA LAB
Home
Lab Manual
biological reagents
Cell Free Systems
DNA
In Vivo Systems
misc methods
overexpression
recipes
RECIPES > IN VIVO SYSTEMS

> Preparation of Stock Solutions for Nitrogen Cavitation of Tissue Culture Cells

    > Protease Inhibitor Cocktail

 

Protease Inhibitor Cocktail

  • Add solutions in the order listed in the following table:
100X
PMSF* 100 mM
Bestatin* (4 mg/mL in methanol) 10 mM
Aprotinin 10 µM
Leupeptin 100 µM
Pepstatin 100 µM
EDTA pH ~ 8 100 mM

* PMSF and Bestatin are used the same day - Therefore make only a small amount

    • Aprotinin, Leupeptin, and Pepstatin might be soluble in water. They should be mixed together and stored in aliquots at -80°C