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RECIPES > TRICINE GEL RECIPE

> Preparation of Stock Solutions Required for Making Tricine SDS Gels

   

> Preparation of Tricine SDS Gels

   

> Preparation of the Stock Solutions Required for Running Tricine SDS Gels

    > Preparing to Run Tricine SDS Gels

Preparing to Run Tricine SDS Gels

  • When you are ready to run your gel, in two beakers dilute cathode and anode buffer stock solutions to 1X.
  • Assemble electrophoresis module with the gel cassettes (refer to the Bio-Rad Mini-Protean® Cell Assembly Guide).
  • Pour cathode buffer into the inner sandwich compartment.
  • Pour anode buffer into the container up to the mark.
  • Place cover and connect electrodes.
  • Program running conditions: 40 min @ 30V (constant voltage) then 1 hr @ 40-50mA (constant current).

Reference:

Hermann Schägger and Gebhard von Jagow, " Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa", Analyt. Biochem. 166, 368-379 (1987)